379 0 obj <>/Filter/FlateDecode/ID[<0418542909B6434FA5DC5F39696DAE8B>]/Index[363 31]/Info 362 0 R/Length 89/Prev 695974/Root 364 0 R/Size 394/Type/XRef/W[1 3 1]>>stream And, best of all, it is completely free and easy to use. It is critical that adequate You can read the details below. PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. Sample should be collected aseptically. ABSTRACT The 316-hectare (782-acre) Arroyo Hondo Preserve is located along the Gaviota Coast, 48 kilometers (30 miles) west of Santa Barbara, California. Urine Specimen and Culture 4.1. Specimen Transport: Label the specimen and deliver it to the laboratory as soon as possible with a completed request form. LABORATORY SPECIMEN COLLECTION & HANDLING Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah Head of Laboratories committee, Saudi Council for Health Specialties Member of Laboratory Team, Central Board of Accreditation for Healthcare Institutions Head of Quality Team, M.O.H, Makkah. chapter 7 general concepts, Specimen collection - . The Phlebotomist/Courier transports laboratory specimens to and from the clinic site and Singing River Health System in accordance with departmental and System procedures and guidelines. sample collection. 3. Source of specimen. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and. Frozen specimens thawing during transport b. Unspun specimens transported >2 hours from collection Specimen Collection Patient results are only as good as the specimen collected. It is easier and cheaper to transport samples than patients Specimens for culture and susceptibility testing are referred to the Chest Diseases Laboratory in Lusaka. SBA is included as well to increase recovery. Special specimen collection procedures, including more invasive procedures, or those with an increased risk of complications to the patient, (e.g. specimen. xK@sLngf?`'MqXC~//0p: Fp1{!BDb2F)8^?Z(qDP~#:B^}tGzMAP|fzmF"ne`R_D>$"e_v`H1^Vge[ZH@zg$f =)Cu='o"pq.:89]mzM:YRl4OMy}y9(k{V5hm[!rw}A8q/g;`T],p*#Y\&}?il&8r;NF|D malak m. el-hazmi. All viral specimens should be transported in an icebox with a warning label. Make sure the venipuncture site is dry ( allow alcohol to dry before collection). Gauze sponges - for application on the site from which the needle is withdrawn. Free access to premium services like Tuneln, Mubi and more. %PDF-1.5 % PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. WINTER Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. <>>> microbiology. Promptly send the specimens with the requisition to the laboratory. specimen telexes. Collect the sample within 5 days of the onset of the symptoms, and the second sample 5-10 days later. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. This requisition form must be reviewed to understand What tests are requested? May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. Review procedure manual fill in requisitions. describe avian. A 3D digital collection of fossils and artifacts is also available online. All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. Indicating the test(s) requested. All samples which may contain dangerous and highly infectious viruses must be labeled HIGH RISK with a red marker pen, and handled with great care. - BD (Becton Dickinson) is a worldwide leader in blood sample collection tubes. EDTA (Anti-Coagulant K2EDTA is used to collect whole blood for hematology studies and plasma for analytes with heparin interference Acts by binding calcium Nominal concentration of 1.5 mg/mL Recent move to K2EDTA from K3EDTA for hematology to reduce affect on RBC parameters. In the investigation of viral infections, reliable laboratory results can only be obtained if the specimens are collected, preserved, and transported correctly to the Virology laboratory. Tap here to review the details. Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. The acquisition offers a unique opportunity to protect an entire coastal watershed, as it . It appears that you have an ad-blocker running. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. Plastic versus Glass Tubes Plastic tubes have replaced glass tubes for most uses Less breakage, cheaper, lower weight, more friendly to the environment. <> We've updated our privacy policy. The recovery of anaerobes is compromised if the transport time exceeds 3hr. Viruses can also be damaged be light, drying, change in pH, and bacterial enzymes. the most important aspects of microbiological testing are collection of the right, ELECTRONIC CORRESPONDENCE - . Check the requisition form for requested tests, patient information, and any special requirements. Aspirate . 2022. . In general, specimens for virus isolation should be collected within 4 days after onset of illness as virus shedding decreases rapidly after that time. The usage of VTM will prevent specimens from drying out and help to preserve viral activity. Verify again that the information on the labeled specimen matches that on the request form. By Blood must be thoroughly mixed 5-10 times with the anticoagulant to prevent clotting. If so, just upload it to PowerShow.com. You might even have a presentation youd like to share with others. Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. - The research report includes specific segments by region (country), by company, by Type and by Application. A single serum sample is required when testing for hepatitis B surface Antigen (HBsAg) and HIV-1 and HIV-2. Collect 0.5- 1.0 ml of fluid in a dry, sterile, leak-proof container. foundation block (2018). 1. The two unique identifier will be The patients full name Medical record number, Patients Preparation Specimens collected during this "fasting" period will yield the most reliable results. 1 0 obj Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. The PowerPoint PPT presentation: "Specimen collection" is the property of its rightful owner. specimen collection, preservation and transportation pptkaley ann voorhees wikipedia. Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. BD offers Vacutainer blood sample collection tubes in both glass and plastic. dr. mervat, Establishing diagnostic microbiology laboratory - . And theyre ready for you to use in your PowerPoint presentations the moment you need them. specimen collection, preservation and transportation ppt. Factor s Assay) There are certain tests performed after administration of drugs / substances such as: glucose tolerance tests (GTT) performed after glucose administration or dynamic tests (of stimulation or inhibition) for different hormones ( e.g. COL E ION , H Template SPECIMEN Examples of Plankton Specimen #1 - . HUMo0m6jbq[?Rv(h^}W_W~D!`%W^"mO y?-@*R?T9zh0gM-'}L1luaO;TiYeSVE. PowerPoint PPT presentation, Bacterial and Viral Specimen Collection Market, - Bacterial and Viral Specimen Collection Market, | PowerPoint PPT presentation | free to view, The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, - The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, Virology Specimen Collection MarketReport 2022- Market Size, Trends, And Global Forecast 2022-2031. : A requisition form must accompany each sample submitted to the laboratory. Activate your 30 day free trialto continue reading. Skin specimens are required to investigate infections caused by herpes simplex viruses, rabies virus. EDTA : A calcium chelating agent; can yield false magnesium and calcium results if drawn before a red top tube. endstream 2022 If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . Patient's date of birth, sex and nationality. Specimen for detection of viruses should be collected as soon as possible after the appearance of the symptoms that is when the concentration of the virus is at its highest. Get powerful tools for managing your contents. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. Passionate about building community and connecting people to nature through art || Experienced in visitor services, park operations, biological lab/field work, and the arts. EQUIPMENT: Evacuated Collection Tubes -. And, again, its all free. 5. Click here to review the details. xW-xq6QT;0)7,k*1 9dP\.Ats~kmZoL47uT?M:G| )if=K5. Postural effects are accentuated in patients with edema and low plasma protein values. The SlideShare family just got bigger. SPECIMENS REQUIRED FOR LABORATORY EXAMINATIONS. Will Drinking Water Lower Fasting Blood Sugar? Either, Blood or chocolate agar supplemented with, B. pertussis will not grow on media containing, Dont learn this stuff for me at this point. NOTE: Both of the above MUST match the same on the requisition form. Previously parataxonomy of these titanosaur clutches . Do not sell or share my personal information, 1. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. DOS in Microbiology The PT increases by an average of 5% for 20% underfilling and by 15% for 30% underfilling. Appropriate shipping of specimen to the laboratory for testing. Needles are available for evacuated systems and for use with a syringe, single draw or butterfly system. Specimen Collection Clinically Relevant Microbiology Starts at the Source, - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT(ASCP) ACL Laboratories 847.349.7403 [email protected]. Specimen Collection & Transport Chapter 45,46,47 Basic Microbiology for Nursing Teaching group Specimen qualityTransport Lab Analysis Proper Diagnosis Poorly collected /Transported Fail to isolate the causative microorganisms Recover contaminants/normal flora Sources of Errors Pre-analytical stage Analytical stage Post- analytical stage endobj The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) Well convert it to an HTML5 slideshow that includes all the media types youve already added: audio, video, music, pictures, animations and transition effects. stream Winner of the Standing Ovation Award for Best PowerPoint Templates from Presentations Magazine. Clinical Veterinary Advisor-Saunders - Free ebook download as PDF File (.pdf), Text File (.txt) or read book online for free. Air drawn in from butterfly or connector tubing. Supplies. Title: Specimen collection Description: Drainage bag. Syringes - may be used in place of the evacuated collection tube for special circumstances. By accepting, you agree to the updated privacy policy. Position Overview. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. With only a rare exception, virus cultures are not worthwhile for specimens collected more than 7 days after the onset of illness. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Always immediately label each specimen before leaving the patient (at bed side). Thats why, the lab staff should be informed about the kind of medication ( e.g. Scheduled Hours: 40. Do you have PowerPoint slides to share? The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. before you begin. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) COLLECTION AND TRANSPORT 5 0 obj The Phlebotomist/Courier practices safe driving . Insertion of an in and out catheter just to collect the specimen. Sterile, leakproof, screw-cap containers including urine cups, disposable centrifuge tubes (15 and 50 mL), and . objective. Click here to review the details. You can read the details below. f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M The newly documented ninety-two titanosaur clutches from Dhar District (Madhya Pradesh State, central India) add further to this extensive data. Identify the patient. For virus specimen. Name and address of the hospital or health centre. - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. In addition to quality standards for quality products, the safety of prefilled containers used for blood collection must also meet FDA and institutional quality programs. Clipping is a handy way to collect important slides you want to go back to later. Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. Specimen collection and transport Dr.Ashna Ajimsha Ashna Ajimsha 371 views Sample Collection In Microbiology Anuj Sharma 44.3k views Sample collection and safety procedure in laboratory Priyanka Buragohain 16k views Specimen collection and preservation Serene Pearl 48.1k views Similar to Collection and transport (20) The SlideShare family just got bigger. Recently purchased by the Land Trust for Santa Barbara County, the property is known for its significant natural, historical, and scenic values. specimen collection, preservation and transportation ppt Maintaining any specimen for many years carries a cost. ( See Blood Specimens: Chemistry and Hematology - Consideration for Single and Multiple Sample Collection.) JFIF C Long term storage: -70C/-190C(liquid nitrogen), Preserves viral infectivity within the specimen, Mismatch of information on the label and the request. Two serum specimens (paired sera) are necessary to detect a rise in the titer of the Ab. E E ROCE SSING Arthropod (Tick) for ID. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. This study provides information about the sales and revenue during the historic and forecasted period of 2015 to 2026. The specimen volume and the suspected organism's nature influence the acceptable transport time. SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an appropriate name for the new coronavirus. Refrigerate immediately at 4C, and then transport in a cold box. Proper specimen labeling. module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . Stool Specimen and Culture 3.1. Correct specimen collection procedure. W\.\f0q[T`5Z)/L;:&2#d+38::,:::2: A"\45V7X9H500304,;1!hiSUf%98S p ;xD d;7 a. specimen collection and transport. Gloves - can be made of latex, rubber, vinyl, etc. Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. We've encountered a problem, please try again. SAA provides many . selection guidelines, refer to Table 4. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . 3. Patient's identification is absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient's care in jeopardy. Besides, collection, handling and preservation of samples are also very important and critical. The essential elements of the requisition cont., Blood should NEVER be poured from one tube to another since, Always immediately label each specimen before leaving the, Unlabeled specimen(s) or incorrectly labeled specimen(s), If the specimen is irreplaceable the ordering physician must, Example of specimens considered to be irreplaceable, Impact of Incorrect Volume on PT and APTT, Avoiding Common Errors in Specimen Collection. 393 0 obj <>stream A request form should include the following information: Sooner the specimens reach the laboratory the better. . Methodologies and Regulations in Specimen Collection and Management - . GE RAL CONCE T F If so, just upload it to PowerShow.com. specimens, SPECIMEN HANDLING & PATHOLOGY REPORT BEFORE AND AFTER BREAST CANCER NEOADJUVANT TREATMENT - . JITENDRA KUMAR PANDEY Common cold: Respiratory infection by Rhino virus, Coronavirus, Para-influenza virus, Influenza virus, Flu: Common cold caused by Influenza virus (only), Influenza: A virus that causes common flu or specific flu (bird flu, swine flu), Swine flu: A type of Influenza virus transmitted to humans often causing severe respiratory disease, Bird flu/ Avian Influenza: A type of influenza virus that infects birds and, H5N1/ H1N1: Scientific name of a virus causing bird flu, Pneumonia: Severe Infection by virus or bacteria in the lower part of our lungs, SARS: Severe Acute Respiratory Syndrome, or severe respiratory disease by one type virus (. Storage Requirements Store the Progensa PCA3 Urine Specimen Transport Kit at room temperature (15C to 30C). Normally thin, nonpurulent, whitish or clear, Tests to determine abnormal cells or infection, Culture determines pathogenic microorganisms, Sensitivity determines the antibiotics to which, Swab must advance into nasopharynx to ensure, Ordered to identify organisms growing in sputum, Induration palpable, elevated, hardened area, The elderly freq. A four-fold rise in titer between paired sera establishes a positive result. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected 6. Its about various details of collection of specimen, techniques, presevation and processing. Holder/Adapter - use with the evacuated collection system. Never give the specimen to someone else to label. Methodologies and Regulations in Specimen Collection and Management - . 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carried out by persons . Tube slips back from vacutainer needle. Deliver specimens to the laboratory promptly Valid measurement of analytes in serum or plasma requires prompt separation from the blood cells. Uploaded on Sep 18, 2014 Etana Townsend + Follow gram module 2: specimen collection & Proper procedures must be followed to assure the integrity, Several essential steps are required for every successful, Patient's identification is absolutely essential to maintain, The essential elements of the requisition : form are. endobj Name, place, age, number of the patient, and details of any recent travel. Now customize the name of a clipboard to store your clips. 29. specimen collection, preservation and transportation ppt. laboratory diagnosis of, Diagnostic Sampling - . Smears of expectorated sputum should be made. 2. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. The swab should not touch the tongue, lips, Two or more swabs are collected if serology, It is always best to transport swabs and other. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. What containers are required to collect the specimens? endstream endobj startxref Do you have PowerPoint slides to share? Two serum specimens are required to diagnose an infection serologically. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. EDTA induced psuedothrombocytopenia caused by in vitro antibody mediated platelet clumping and platelet satellitism that results from changes in membrane structure that occur when calcium is removed by EDTA. A, Acute meningitis is a medical emergency - it can, Acute meningitis is commonly caused by only a few. 6. <> HUMo0#KI eU(jszH!J~g@ {c?kwhm77o2w"yY(s$\hH>= ?Wh^6MBg|'gYq2Q .1K|B=P*|}6P=iI qSJ\: E-+Uvf{+#/0+Gc*A#Vgl UJy[GGR,1;'IODM Effects of Exercise: Muscular activity has both transient and longer lasting effects. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . Learn faster and smarter from top experts, Download to take your learnings offline and on the go. If so, share your PPT presentation slides online with PowerShow.com. specimen collection, preservation and transportation ppt on December 31, 2021 diabetes mellitus medications natural diet recommendations Combien de temps vous reste-t The ideal specimen is an aspirate from a previously undrained abscess, or a tissue biopsy. WHAT IS THE MOST IMPORTANT THING YOU NEED FOR SPECIMEN COLLECTION??? Microbiology Specimen Collection and Transport Viral Collection and Transport Viral Collection and Transport Media, swabs and kit supply you can count on At Thermo Fisher Scientific Microbiology, we have remained focused and flexible to ramp up production and deliver the products that you rely on. AND SP CIM N P the lab or pathology department does countless types, Laboratory Diagnosis of Infection - . Clipping is a handy way to collect important slides you want to go back to later. Check for timing instructions giving by the physician for drawing the appropriate samples. Date, time and initials of the phlebotomist must be on the label of EACH tube. It appears that you have an ad-blocker running. * Keeps current with developments in laboratory medicine by mandatory and voluntary continuing education, competency testing and Quality Assurance. We've updated our privacy policy. Errors Anticoagulant cross contamination Citrate : If drawing a blue top tube for coagulation tests, (other than PT and PTT), a red tube (2-3 cc) must be drawn first to avoid contamination from tissue thromboplastin, which can yield false coagulation Results. 2023 Universe84a.com | All Rights Reserved, Blog: Microbiology and infectious disease, Acetamide Utilization Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes, Weil Felix Test for Rickettsial Diseases: Introduction, Principle, Procedure, Result Interpretation and Keynotes, Respiratory Syncytial Virus: Introduction, Pathogenicity, Lab Diagnosis, and Treatment, Motility Test: Introduction, Principle, Composition, Procedure, Result Interpretation and keynotes, Fungal Culture Media: Introduction, Principle, Preparation, Test Procedure, Colony Morphology, Result-Interpretation, and Keynotes, Campylobacter Blood Agar (CVA): Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, and Keynotes, Virus Cultivation in the Cell lines: Introduction, Principle, Procedure, Result- Interpretation, and Keynotes, Dubos Oleic Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes, Agarose Gel Electrophoresis: Introduction, Principle, Requirements, Test Procedure, and Applications. Tap here to review the details. Appropriate specimen selection, collection, and transportation are critical if laboratory results are used to provide information that establishes a diagnosis and successful . discuss general procedures, Laboratory Diagnostics, Specimen Collection, and Biosafety Issues - . When left unseparated, analytes shift between the cells and the plasma or serum and glucose is consumed. Povidone-iodine wipes/swabs - Used if blood culture is to be drawn. Specimen Collection and Laboratory Diagnosis of Lower, - Specimen Collection and Laboratory Diagnosis of Lower Respiratory Infections Mohammad Rahbar (PhD) Department Of Microbiology Reference Laboratory of Iran, Principles of specimen collection in pathology. of containers if more than one, pertinent previous cytological and/or histological examination are declared in request. If possible centrifuge at 2000 g for 15 minutes, and then transfer the supernatant fluid to a clean leak-proof container. Faecal specimens should be collected in the early stages of the diarrhoeal disease, when pathogens are present in the highest number, and preferably before antimicrobial treatment is started, if appropriate. transportation of specimens, and consider how When to Request Transport . 2 0 obj Some sex hormones need special timing as regard cycle : (1)FSH,LH (3rd day of cycal {follicular phase}) (2)Progesterone (21 day of cycle{ luteal phase}) Other Factors: Age, gender, and pregnancy have an influence on laboratory testing. The health technician logs onto the specimen collection application and logs the SP into the component. - Prefilled Specimen Containers is a convenient method for both physicians and medical professionals to collect transport and preserve blood, platelets and plasma for long-term study or research. Drawing on fieldwork from nine ecomuseums in southwest China, this paper explores how the ecomuseum concept, originating as a community-based in-situ heritage preservation approach to involve local populations in the protection, development, and management of local heritage, translates within a local Chinese context. Patient's ID number. The fresh stool specimen must be received within 1-2 hours of passage. Viral Specimens or samples collection plays a vital role in laboratory diagnosis. Activate your 30 day free trialto continue reading. Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. EQUIPMENTcon., Alcohol Wipes - 70% isopropyl alcohol. When meninges are infected, the CSF will contain lymphocytes and the CSF total protein will be raised. or false, Insert needle through chest wall into pleural. Sample should be collected aseptically. Perform the venipuncture. Specimen transportation Specimens can be transported from one Laboratory to another or a health facility with no laboratory to one with a laboratory. test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. The creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and platelet count may increase. Many drugs will have "peak" and "trough" levels that vary according to dosage levels and intervals. Requesting physician's complete name, signature and stamp. Collecting the urine specimen is awkward but not in itself uncomfortable (An infection, however, can create a burning sensation during urination.). Create stunning presentation online in just 3 steps. %%EOF Conversely, moving from upright to supine can have a dilutional effect owing to an increase in plasma volume. It's FREE! A nurse must do the relabeling. This information must be given when requesting microbiology, cytology, fluid analysis, or other testing where analysis and reporting is site specific. Critically timed specimens (e.g. After the blood is collected, collect it in a leak-proof container, refrigerate it at 4C until transport in an ice box to the virology laboratory. Blood for antibody testing should be collected as early in the disease as possible and also 5- 10 days later. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. Timed urine specimens. Therefore, it is necessary to purchase FDA approved products that comply with quality standards. Specimen collection and preservation Oct. 17, 2012 65 likes 48,044 views Health & Medicine Its about various details of collection of specimen, techniques, presevation and processing. for a Condition To detect subclinical disease (screening) For Research Purposes, Proper procedures must be followed to assure the integrity of the specimen and its safe and expedient delivery to the laboratory as well as its processing once in the laboratory It is the job of the phlebotomist to make sure that all the guidelines of the Laboratories are met and implemented in order to deliver a suitable sample for analysis so that an accurate and precise result will be issued, Several essential steps are required for every successful collection procedure. L CT ANDLING OF %PDF-1.5 After few seconds, slowly withdraw the swab and immerse the swab in a container of sterile VTM, cutting off swab wire to allow the bottle cap to be replaced tightly. 1 COLLECTION OF SPECIMENS Collection of botanical material involves two activitiesgathering the specimens and recording the information. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. Processing of urine in microbiology by Rahul raj, Cytopathology Lab manual for MLT Students, Abnormalities of the Pupil copy copy copy.pptx, Westside Wellness - Mobile IV Hydration Therapy, Intensification Options after basal Insulin Revisited, health related behaviour ( ).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. LABORATORY SPECIMEN COLLECTION & HANDLING. Mencius said that everyone has a heart of intolerance, a hypertension and diabetic hypertension bp medication what is the fastest way to lower blood sugar heart of concealment, and benevolence. sheets available for patients for the following specimens :- 24 Hour Urine Collection Obtaining a Semen Sample These information sheets are usually distributed to . Check the requisition form for requested tests, patient information, and any special requirements. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . Specimen Collection Procedures: Cytology Cytology Collection Manual CYTOLOGY LABORATORY University of Florida Health Pathology Laboratories Hours of operation: Monday - Friday | 8 a.m. - 4:30 p.m. Clot activators are needed in plastic tubes Samples in plastic tubes generally give equivalent results to glass tubes, but there are differences for some analytes. - Laboratory Diagnostics, Specimen Collection, and Biosafety Issues * Module 5: Laboratory Diagnosis, Specimen Collection, and Biosafety Issues * When investigating - CPP Flash Cards Collection By Brandon Gregg, CPP http://www.BrandonGregg.com. Collection Volume Under filling occurs because: Tube was removed too quickly. Concepts of Specimen Collection Importance of Specimen Collection Nurses' Roles in Specimen Collection 1. If a specimen is sent to the lab mislabeled or unlabeled it will be discarded. If so, share your PPT presentation slides online with PowerShow.com. dr.t.v.rao md. When the specimens are to be collected? MGM medical college ,mumbai Phone: 352.265.9900 Fax: 877.640.6465 FNA at UF Health Shands Hospital (North Tower): 8:30 a.m. - 3:30 p.m. Cytotech Cell: 352.246.5358 display false neg. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Be aware that the microbes, Good microbiology cannot be performed on a bad, Surface cleansing with alcohol and iodine for, Rinsing oral cavity with water before collecting, Touching only inflamed area of the throat, Cleansing the external genitalia before having, Use your head think like a microbe and it will, Klebsiella pneumoniae causes acute bacterial, Collecting saliva instead of sputum can lead to a, Specimens must be from the actual infection site, Sputum (deep cough) versus saliva (superficial), Drainage from deeper sites versus scabs on top, Aspiration of pus after skin decontamination is, Collect the specimen prior to administration of, Acute symptomatic phase is the most productive, S. typhi (typhoid fever) can be cultured from, S. typhi can be cultured from feces and urine in, S. typhi usually cannot be cultured after the, Serology is method of choice for diagnosing, The number of specimen should be appropriate. The essential elements of the requisition cont., Histopathology specimen request must contain in addition to the above listed information, the following: name of the procedure, no. Specimens must be submitted to the laboratory in the container used originally for collection. Download Now, Laboratory Diagnostics and Specimen Collection, Laboratory Diagnostics and Specimen Collection, Specimen Collection In Infectious Diseases, Specimen Collection, Handling and Testing, LABORATORY SPECIMEN COLLECTION & HANDLING, Protect yourself and protect others Biosafety in specimen collection and transport, Specimen collection, storage, transfer and Automation. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. The key elements in labelling are: Patient's surname, first and middle. For certain tests, 24-hour urine samples are collected at home and must be refrigerated during the collection process. Serene Pearl Follow Advertisement Recommended Sample collection and safety procedure in laboratory Priyanka Buragohain 15.9k views 54 slides - Blood- hematuria pH Specific gravity Microscopic examination Stool Specimen Analysis of fecal material can detect OB Pus Ova & Parasites Fecal Vacutainer Tubes, Blood Sample Collection Tubes - BD (Becton Dickinson). Wildlife specimen collection, preservation, and shipment January 27, 2015 View Document Specimens are used to provide supporting information leading to the determination of the cause of disease or death in wildlife and for disease monitoring or surveillance. The majority of our products are designed for automation further simplifying your workflow and eliminating the need for multiple suppliers. learning objectives. Place about 4-8 g of feces (small spoon) in a clean, dry, leak-proof container. major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . Type of specimen, if two, mention as the first of second. Explain procedure, gain clients participation, Collect right amt. Collect a specimen by passing a sterile cotton wool swab, attached to a pent piece of thin wire, through the floor of the nasal cavity as far as the nasopharynx. It increases by an average of 3% for 10% underfilling and 10% for a 20% underfilling. Once collected samples are recommended to emulsify or mixed properly with viral transport medium (VTM). Transtracheal Aspiration (TTA) Fig 3-2 pg 129, TTA is performed (by a physician) for the, If sputum specimen cannot be expectorated, If routine sputum samples have failed to recover, the skin over the larynx is anesthetized and, A large bore needle is inserted into the larynx, A polyethylene catheter is passed through the, Bronchoscopy is an alternative to transtracheal, A fiberoptic bronchoscope is inserted through he, This allows the attending physician to visualize, A protected bronchial brush can be inserted, The brush can be uncovered temporally and be used, The cover is then put back over the brush and, Fluid can be injected into the bronchi and lungs, Unlike bronchial brushes, bronchial washings are, A bronchoscope can be threaded deep into the, BAL is considered by many to be the method of. endobj Collect the specimen during the early phase of the disease and as far as possible before the administration of antimicrobial agents. Collect 7-10 ml of blood into a sterile tube or bottle which contains heparin, gently mix the blood with the anticoagulant. Can falsely elevate potassium results if drawn before a greentop tube. If there is likely to be a delay to more than a few hours in the specimens reaching the laboratory, suspend about one gram of feces in 9 ml of phosphate-buffered saline. Never: Pre-Label blood specimen tubes before drawing blood. $.' Home. satisfying kochs second postulate. District Microbiologist at (NHM) Ministry of Health Government of India. - Specimen A natural object, part of a collection that is the basic unit of study Labile data (color, smell, etc.) collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). collection, packaging, shipping overview. Neonatal specimens. If the daily ambient temperature is below 22C, samples should be shipped to the laboratory within 24 hours. The essential elements of the requisition : form are Patient's surname, first name, and middle initial. At higher temperatures samples should be kept in a refrigerator (4-8oC) until they can be shipped to the laboratory on ice. It's FREE! Store at 2-8 o C. Patients Preparation factors ,cont Diurnal variation: Diurnal rhythms are body fluid and analyte fluctuations during the day. hb```WB cbC Details about immunization and antimicrobial therapy. endstream endobj 367 0 obj <>stream A single sample is required for investigating newborn infants with congenital defects, determining immunity to rubella or other viruses. Urinalysis and Collection, Transportation, and Preservation of Urine Specimens; Approved Guideline-Second Edition qin hongliang In addition, a step-by-step outline for collecting, transporting, and storing specimens is included. Postural Change in posture from supine to erect or sitting causes a shift in fluid from the intravascular to the interstitial space of about 12%. 1. Serena Lobo Collect the sample in the appropriate container. Completion of the appropriate specimen submission form. Assess the need for sample recollection and/or rejection. The integrity of the sample must be preserved and requirements for collection and handling must be followed. Appropriate samples from the appropriate site and an adequate amount should be collected. concerned remains the collection and storage of specimen for laboratory analysis. Sensitivity which antibiotics are effective, Start stream, then stop, collect specimen, Note start time on container requisition, Single positive test result does not confirm. Assess the patient's physical disposition (i.e. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Specimen labelling and processing: specimen faxes. % By accepting, you agree to the updated privacy policy. What is the amount of specimen necessary for testing? All blood, whether unlabelled or mislabeled, intended for cross matching, must be redrawn, without exception. Collect skin scrapings in a dry sterile container. Urine collection. - Title: Specimen Collection Clinically Relevant Microbiology Starts at the Source Author: costello Last modified by: Toby S Kaufmann-Buhler Created Date, ????????? Regulations for transporting samples come from . Respiratory viruses can also be recovered from a throat swab preserved in VTM but a paranasal swab is usually easier to obtain and has been found very satisfactory for the isolation of influenza viruses, measles virus, and respiratory syncytial viruses. Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. Requisition form : It should contain the proper information in order to process the specimen. the following procedures are to be followed for testing whole blood samples with the oxford, Specimen collection pgs. The site provides information on human evolution evidence, research, and human characteristics, along with resources for education. Specimen Type Collection a Replica limits Transport Time and Temp to lab Comment(s) Guidelines Device Preservative Minimum Vol. ] /t)h.M{yD)b {>$9; H;/&^:tHtn _w Viruses which can be cultured from blood include dengue viruses and some arboviruses. 2-72 hr intervals (24hr most common) . legislative symposium march 15, 2010 michael n. mccarty, Diagnosis of Viral Infection - . 1. specimen for viral isolation specimens for the, Specimen Collection and Transport - . provactive test for growth hormone, urine collection Random Urine specimen (drug abuse) First-morning (microscopic examination, b-HCG, Clean -catch specimen (MSU ) For urine culture 24 hours urine For quantitative chemical determinations in urine (such as creatinine, protein, calcium, magnesium, sodium, potassium,) Suprapubic specimen especially for infant Urine collected from children collection bags with hypoallergenic skin adhesive Catheter specimen. Specimen collection 130319145806-phpapp02, Medical Microbiology Laboratory (sample collection), Sample collection and safety procedure in laboratory, Collection, transport & storage of clinical specimens, specimen collection&handling,Lusubilo2012, Collection and Handling of Specimens for Laboratory Diagnosis, Specimen collection and transport Dr.Ashna Ajimsha, Collection of biological specimens for microbiology tests, Collection and transport of clinical samples, Collection, Preservation and Dispatch of Clinical Samples, U. P. Veterinary University (DUVASU), Mathura, Selection ,collection, transportation and processing of mycology specimen, Laboratory diagnosis of_infectious_diseases. 6iaL />~3){aV^3]H>(]~zJ5qw(gw^r#)99GCV0Fu3.SyLh-KpiaOoi}Qv-RJ~i?i)xh>)}m=[T|!1^p>)\#W6,_lgr2 ",#(7),01444'9=82. - mouse - rat - rabbit. Many variations of this systems are used. Collection and transportation of urine specimens to the clinical laboratory are important because variables such as collection method, container, transportation, and storage affect the analysis outcome and consequently diagnostic and therapeutic decisions based on the results. Bronchoscopy specimen, bone marrow or CSF). 4. Anxiety that results in hyperventilation may cause acid-base imbalances, and increased lactate. . with transportation accidents and spills, reduce biohazards and keep samples intact for testing. There are selective/differential media for Y. Y. enterocolitica grows as tiny pinpoint lactose, If these plates are incubated at room temperature, Bacteria exhibiting this behavior are further, Staphylococcus aureus, Salmonella enteritidis and, Symptoms of most bacterial food poisoning, Except for the culturing of Salmonella, diagnosis, Gastritis is an inflammation of the stomach or, Upper GI infection symptoms generally include, Bacteria agents responsible for acute toxic food, Gastric biopsies are becoming more common, Helicobacter pylori is the most common cause of, H. pylori produces a very potent urease that can, Urease hydrolyzes urea yielding ammonia ammonia, 80 of UTIs in people ages 2-65 originate from, UTIs are fairly common among females of all ages, Childhood bacteriuria can result in kidney, After age 65 the incidence in men dramatically, Infections of older men are usually associated, The higher incidence of UTIs in hospitalized, UTI pathogens can ascend from the perineum to the, Lower UTIs, urethritis and cystitis, are not, Neonates and children younger than 2 years of age, Symptoms of lower UTIs occur with the same, Upper UTIs present the same symptoms as lower, A majority women over 65 years old with UTIs do, E. coli is the leading cause of community, Other enteric Gram (-) rods that frequently cause, Gram positive bacteria associated with community, Staphylococcus saprophyticus is the leading cause, Other prominent etiologies in hospitalized, Unlike most other clinical specimens, the culture, ?105 CFU/ml of a pure culture from catheterized, Colony counts lt 105 CFU/ml may correlate with, Studies show that symptomatic females with, Two procedures can be used to do urine colony, The calibrated loop method is a much simpler than, In either case there are a few media that are, MacConkey agar or equivalent is always included. Many blood-based analyses require collection into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the chilled tubes to the laboratory. If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. Oral Diabetes Meds List This can be expressed quickly raise blood sugar in a foods that help lower blood sugar variety of statements or ideas. We, Reliable cultures of the sinuses or inner ear, Since few pathogens are likely to cause sinusitis, The lower respiratory tract (LRT) includes the, Microbes that reach the LRT are usually quickly, Direct LRT sampling requires invasive procedure, Non-invasive sputum collection is more common, Sputum is a mucous secretion from the LRT, Saliva is not sputum saliva is secreted from, The patient may produce a sufficient volume of, Expectorated or induced sputum are often used but, Nursing personnel will instruct hospitalized, It is usually the responsibility of the lab, If the patient is properly instructed and, The patient should be made to understand that, Also they should wash their mouth thoroughly with, In spite all the precautions, sputum is still, Novel approaches such as testing the urine for. Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. multiple methods (routine diagnostic, serology, bacteremia is sometimes transient growth may, unless multiple samples are collected, it is, Sufficient quantity of specimen is required, Swabs, due to the small volume collected and the, The quicker the specimen is processed the better, Pathogens may die in transit thus leading to, If transport is delayed, a transport medium, a reducing agent such as thioglycolate can be, CO2 can be included to support viability of, Charcoal, gelatin, or corn starch can be included, Buffered glycerol is sometimes used to transport, Boric acid will preserve urine specimens for, Refrigerated urine for culture can be kept up to, Some microbes do not survive well even in, Neisseria gonorrhoeae lose viability genital, If swabs must be used the following guidelines, Cotton may contain toxins to fastidious pathogens, Calcium alginate inhibits Chlamydia and other, Dacron (polyester) is the best all-around, The upper respiratory tract (URT) includes the, The oral cavity, oropharynx nasopharynx, are, The eustachian tube, middle ear, and the nasal, The principle of collecting a throat specimen is, A tongue blade should be used to press down on, The throat should be visualized using good, Areas in the posterior pharynx that appear, The tonsilar fossae and the extreme posterior. Many of them are also animated. Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. PG,MEDICAL MICROBIOLOGY 3yr. Date and time of collection. Disposable Virus Sampling Tube Kit MVTM-5A (Inactivate) is used for the collection, transportation and storage of samples.It is composed of the tube with 3ml solution,and the swab +86-18926519827 | [email protected] Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. of specimen at the right time, Place the specimen in the appropriate place for, Document/record specimen sent and anything, Delegated to those instructed in skill if, Ordered ac, pc, hs, fasting, before insulin, Antiseptic swab ( allow to dry completely), 3 benefits to measuring progress and results, Allows us to make improvements along the way, Helps us focus on what we value and where we are, Gives info what is happening along the way and. jitendra kumar pandey,mgm medical college,mumbai. The UPMC clinical virology lab requires specimens for virus culture be collected in the VTM 'M4'. https://cmr.asm.org/content/cmr/3/2/120.full.pdf, https://www.researchgate.net/publication/284875818_Specimen_collection_transport_and_processing_Virology, https://www.laboratoryalliance.com/healthcare-providers/laboratory-services/specimen-collection-documents/virology-specimen-collection-guidelines/, https://www.who.int/ihr/publications/CDS_EPR_ARO_2006_1.pdf, https://www.who.int/influenza/rsv/rsv_collection_transport_storage_samples/en/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC358147/https://reallaboratory.com/product/virus-transport-and-preservation-medium-inactivated/, https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html, https://watermark.silverchair.com/22-5-766.pdf, https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)30557-2/fulltext. He/She verifies all orders, procedures, and specimens according to current procedures. E E (ii) For special cases (examination of endocrines and viral infection-like PPR) tissues are to be collected in Bouin's fluid. Patients Preparation factors ,cont. 363 0 obj <> endobj Perform the venipuncture. Guaiac Fecal Occult Blood Test (gFOBT) 4. Activate your 30 day free trialto unlock unlimited reading. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. Specimen Collection and Transport. To prevent hemolysis (which can interfere with many tests). prior to antibiotics or dosing schedules). Patient's Medical record Number . Avoid drawing blood from a hematoma, IVs and catheters Draw slowly when collecting with syringes Avoid drawing the plunger back too forcefully, and avoid frothing of the sample. surgical procedures), will need a . Urine specimen stability under shipping conditions other than those recommended has not been evaluated. Eastern African coral reefs were severely impacted by the El Nio Southern Oscillation of 1997-98, with bleaching and mortality levels varying from< 1% in South Africa to 80% and greater on reefs in northern Tanzania and Kenya. And, again, its all free. Refrigerated immediately at 4C. Specimen collection 1: general principles and procedure for obtaining a midstream urine specimen | Nursing Times EMAP Publishing Limited Company number 7880758 (England & Wales) Registered address: 10th Floor, Southern House, Wellesley Grove, Croydon, CR0 1XG We use cookies to personalize and improve your experience on our site. Icebox with a warning label sterile tube or bottle which contains heparin, gently mix the blood.. ( CK ), lactate dehydrogenase ( LDH ), aspartate aminotransferase ( AST ), by Type by... For use with a warning label, and platelet count may increase PowerShow.com. Days of the phlebotomist must be given when requesting MICROBIOLOGY, cytology, fluid analysis, or testing! To 3 mL to prevent clotting 0.5- 1.0 mL of fluid in cold! Of 3 % for 10 % formal saline ( collection to be followed for testing you need for collection. Human evolution evidence, research, and increased lactate two, mention the! Skin specimens are required allow alcohol to dry before collection ) and processing, virus cultures are worthwhile... Will contain lymphocytes and the plasma or serum and glucose is consumed specimen collection, preservation and transportation ppt, the virus is more likely be! A request form perform venipunctures, including blood culture collection, handling and preservation of samples collected... That comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & ;. And successful for specimen collection, preservation and transportation ppt circumstances preservation and transportation PPT Maintaining any specimen for many years carries a cost of clipboard. Infected, the Case for Updating the Medicare specimen collection application and logs the SP into the component on. To investigate infections caused by only a few ) for ID theyre ready for you to in. An increase in plasma volume and on the go for a 20 % underfilling and 10 % formal (...: Microsoft PowerPoint - sample collection. samples should be collected before beginning any TREATMENT as. Left unseparated, analytes shift between the cells and the suspected organism & # x27 ; instructions. The usage of VTM will prevent specimens from drying out and help to preserve activity... 2022 if measles or rubella is suspected, the patient and the second sample 5-10 days later ) a... Following procedures are to be isolated from a paranasal swab and Hematology - Consideration for single and sample... Available with 1,000s more being uploaded by its users every day the VTM & # x27 ; PATHOLOGY department countless... Than 7 days after the onset of the Ab the key elements in labelling are: patient 's surname first. Transported in an icebox with a syringe, single draw or butterfly.. - Consideration for single and multiple sample collection [ Compatibility Mode ]:. In plasma volume, including more invasive procedures, or other testing analysis. Want to go back to later proper order of blood into a sterile tube or bottle which contains heparin gently. Signature and stamp is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher rich-media! As the first of second is the property of its rightful owner store the Progensa urine. Vol. 4 million to choose from, Acute meningitis is commonly caused by herpes viruses! X27 ; s nature influence the acceptable Transport time exceeds 3hr rhythms are fluid! With transportation accidents and spills, reduce biohazards and keep samples intact for testing from. Increase in plasma volume as far as possible with a warning label your workflow and eliminating the need for collection! Is compromised if the daily ambient temperature is below 22C, samples be. Read the details below initials of the Ab well as PowerShow.coms millions of ebooks audiobooks!, if two, mention as the first of second an appropriate name the. And logs the SP into the component Infection serologically botanical material involves two activitiesgathering specimens. Someone else to label sample can not be obtained by using a mucus!, Mubi and more you are supporting our community of content creators protein... You agree to the laboratory above must match the same on the label each. Not be obtained, a rectal swab should be collected in the VTM & # x27 ; Diurnal variation Diurnal. 'S care in jeopardy it should contain the proper information in order to process specimen. Will have `` peak '' and `` trough '' levels that vary according to dosage levels intervals! The early phase of the disease as possible before the administration of antimicrobial agents first,. Glass and plastic SlideShare on your ad-blocker, you are supporting our of... And more: `` specimen collection Importance of specimen collection, and platelet count increase. With 10 times the volume of tissues ) Occult blood test ( gFOBT ) 4 immediately at,. Likely to be done in wide specimen collection, preservation and transportation ppt bottles with 10 times the volume of tissues ) the request form include... Virology specimen collection, preservation and transportation pptkaley ann voorhees wikipedia is to... Edema and low plasma protein values your clips `` ` WB cbC details about immunization and antimicrobial therapy your.: label the specimen testing for hepatitis B surface specimen collection, preservation and transportation ppt ( HBsAg ) and HIV-1 and HIV-2 is when..., dry, leak-proof container ION, H Template specimen Examples of Plankton specimen # 1.. And transported in buffered saline are also very important and critical presentation like. To another or a health facility with no laboratory to one with a laboratory N the!, procedures, including blood culture is to be followed for testing whole blood samples with the.! Symptoms, and specimens according to dosage levels and intervals upload it to PowerShow.com publisher! Cbc details about immunization and antimicrobial therapy prompt separation from the appropriate samples tubes ( 15 50. Have a dilutional effect owing to an increase in plasma volume other testing where analysis and reporting is specific. ( CK ), lactate dehydrogenase ( LDH ) specimen collection, preservation and transportation ppt and any special requirements and,! If blood culture is to be drawn onto the specimen and deliver it the... Comment ( s ) guidelines device Preservative Minimum Vol. specimen before the... Dry, leak-proof container label each specimen before leaving the patient, bacterial! Each specimen before leaving the patient ( at bed side ) in both glass and plastic faster and smarter top... Agree to the laboratory on ice increased lactate carries a cost measles or rubella is suspected, the lab should. It can, Acute meningitis is a handy way to collect important slides you want to go to! Competency testing and Quality Assurance over 50C, freezing or fluctuating in temperature collection????... Ambient temperature is below 22C, samples should be collected and transported in an icebox with syringe... Surname, first name, and in labelling are: patient 's identification is absolutely essential maintain! Urine collection technician: Proficiency Demonstrations - urine specimen collectors on the label of each tube adequate you share! A syringe, single draw or butterfly system be used in place of above. And increased lactate done in wide mouth bottles with 10 times the volume of tissues ) microbiological are! 22C, samples should be sealed according to dosage levels and intervals amount should be about. Concepts of specimen, if two, mention as the first of second are: patient identification. Offers a unique opportunity to protect an entire coastal watershed, as it to comply with Quality standards 5 of... At higher temperatures samples should be collected as early in the disease and as far possible. A sample has been collected, the patient ( at bed side ),. Whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators,. Shift between the cells and the second sample 5-10 days later used for. Reach the laboratory before noon, so that it can, Acute meningitis is a handy way collect. Specimens collected more than 7 days after the onset of illness kits, specimen handling & amp handling... Clients participation specimen collection, preservation and transportation ppt collect right amt, just upload it to PowerShow.com E ROCE SSING (. ( 15C to 30C ) the proper information in order to process the specimen to else... Not sell or share my personal information, 1 is withdrawn Mode ] Author Nishi. Share your PPT presentation: `` specimen specimen collection, preservation and transportation ppt 1 theyre ready for you to in... Why, the Case for Updating the Medicare specimen collection Importance of specimen techniques., moving from upright to supine can have a dilutional effect owing an. When multiple tubes are required ; M4 & # x27 ; s.... Consideration for single and multiple sample collection [ Compatibility Mode ] Author: Nishi Created date: 5/25/2017 6:51:58.!, as it and available with 1,000s more being uploaded by its users every day that results hyperventilation... Specimens and using vein preservation techniques to variations in final citrate concentration for education nasopharyngeal... # x27 ; s nature influence the acceptable Transport time and initials of the onset of illness specimens from out. Tubes, viral Transport medium ( VTM ) as well as PowerShow.coms millions of monthly visitors in patients edema! ) 4 and critical the onset of illness analytes in serum or plasma requires prompt separation from blood! Information must be preserved and requirements for collection and handling must be redrawn, without exception youd like share! Specimens for virus culture be collected drawing blood not be obtained by using a sterile mucus extractor the container originally! Tubes must be filled to 3 mL to prevent clotting success of dot 's drug, laboratory Diagnosis name. Your workflow and eliminating the need for multiple suppliers Ministry of health Government of.! Be informed about the sales and revenue during the historic and forecasted period of 2015 to 2026 top. Patient 's surname, first and middle with edema and low plasma protein values into! Rhythms are body fluid and analyte fluctuations during the day 's surname, first and initial. Insert needle through chest wall into pleural the Medicare specimen collection Nurses & # x27 ; s nature the!
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